Basic Fibroblast Growth Factor Supports Undifferentiated Human Embryonic Stem Cell Growth Without Conditioned Medium
نویسندگان
چکیده
منابع مشابه
Feeder & basic fibroblast growth factor-free culture of human embryonic stem cells: Role of conditioned medium from immortalized human feeders
BACKGROUND & OBJECTIVES Human embryonic stem cell (hESC) lines are commonly maintained on inactivated feeder cells, in the medium supplemented with basic fibroblast growth factor (bFGF). However, limited availability of feeder cells in culture, and the high cost of growth factors limit their use in scalable expansion of hESC cultures for clinical application. Here, we describe an efficient and ...
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objective: embryonic stem cells (escs) are pluripotent cells capable of extensive proliferation while maintaining their potential to differentiate into any cell type. the therapeutic potential of these cells are promising, but in many cases limited by our inability to promote their differentiation. in this study we examined the effect of inducer factors in direct differentiation of mouse escs i...
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In order for human embryonic stem cells (hESCs) to be cultured on mouse embryonic fibroblast (MEFs) feeder cells, continuous basic fibroblast growth factor (bFGF) supplementation is required. However, the role of bFGF in a culture system using human-derived feeder cells has not been evaluated until now. In this study, we propagated the widely used hESC lines, H1 and HSF6, on human placenta-deri...
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ژورنال
عنوان ژورنال: Stem Cells
سال: 2005
ISSN: 1066-5099,1549-4918
DOI: 10.1634/stemcells.2004-0211